The Brighton Beer Dispensary in Dean Street made the announcement on Sunday and said it would be closed from Monday onwards.
Management cited various reasons for the closure including the rise in energy costs, Brexit and “other issues” which meant they did not expect to survive.
They thanked customers for their support over the years.
In a Facebook post, they said: “We’re very sorry to have to announce the closure of the Brighton Beer Dispensary.
“With the rise of electricity bills, Brexit nonsense and other issues, we just can’t see ourselves surviving.
“We will be closing from tomorrow onwards.
“We’d like to say a huge thank you to everyone that has supported us over the years. We wish you all well.”
Brighton Beer Dispensary was first opened in 2014 by local brewery Brighton Bier in partnership with Late Knights brewery, replacing the Prince Arthur pub.
However, shortly after Brighton Bier left to pursue their own pubs and bars, later opening the Brighton Bierhaus.
After Late Knights was dissolved, the pub was taken over by Southey Brewing which also has locations in Dulwich and London.
People were quick to share their reactions to the news, with hundreds commenting on the social media post.
One woman said: “Very sad guys, I’m sorry we’ve not been regulars for a few years.
“We are all responsible for irregular patronage and our lack of supporting you.
“Thanks for the memories.”
Another wrote: “I’m really sorry it’s come to this, BBD is a great place and I was really looking forward to seeing more of it coming out of the worst of the pandemic.
“Thanks for the memories over the years, it’ll be really sad to see you gone and I hope you can have a bash to go out in style.”
One man commented that it was “a sign of the times” and he fears there will be “many more”.
“Really sad news. A really lovely little pub, really sorry to hear that you’re closing. Thanks for the lovely beers,” said one Facebook user.
Brighton Beer Dispensary have been contacted for comment.